Determination of phosphonylated tyrosine as a marker of exposure to nerve agents in blood plasma dry spots by HPLC-HRMS/MS
T15N3
N.L. Koryagina, T.I. Aliushina, G.V. Karakashev, E.I. Savel’eva, N.S. Khlebnikova, A.S. Radilov
A procedure for the determination of phosphonylated tyrosine, a marker of exposure to organophosphorus nerve agents, in blood plasma dry spot (DBS) samples by means of liquid chromatography/tandem high resolution mass spectrometry was developed. The DBS samples on Protein Saver 903 Card substrates were shown to be stable for 2 months on storage at 22 and 40 оС. It was found that the temperature of storage has a high impact on the stability of the DBS samples: the concentration of the analyte in the samples stored at 40 оС was about 3 times lower compared to the samples stored at room temperature. It was shown that the special Protein Saver 903 Card substrates could be replaced by an ordinary blue ribbon filter paper. In the latter case, the tyrosine adducts of nerve agents were reliably determined in the DBS samples stored for 1 month at room temperature (22 оС). However, for storage at elevated temperatures (40 оС), the use of filter paper substrates is not recommended. The proposed approach to storage of blood samples allows one to take and easily transport large series of samples and automate their analysis.
Исследование методом жидкостной хроматографии/масс-спектрометрии фосфолипидного спектра плазмы крови пациентов
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