Microextraction of aromatic microbial metabolites by packed sorbent (MEPS) from water solutions followed by gas chromatography/mass spectrometric analysis of their silyl derivatives
T14N2
P.D. Sobolev, A.K. Pautova, A.I. Revelsky
The paper describes the results of extraction of aromatic microbial metabolites (phenylcarboxylic acids) from model water solutions using microextraction by packed sorbent (MEPS) followed by the determination of their silyl derivatives with the aid of gas chromatography/mass spectrometry. The developed sorption-desorption, derivatization and gas chromatography determination conditions allows to detect a number of compounds, which are established and prospective disease markers. This detection could be performed at the level of their content in blood of healthy donors (0.5 μmol L–1) and in patients with an initial stage of sepsis (2–3 μmol L–1) and other serious diseases caused by significant microbial load and led to the development of multiple organ failure in intensive care unit patients. Recoveries of phenylcarboxylic acids using nonpolar sorbent (C18) reached 20–65% for hydroxylated acids (phenyllactic, 4-hydroxybenzoic, 4-hydroxyphenylacetic, 4-hydroxyphenylpropanoic, homovanillic and 4-hydroxyphenyllactic) and 100% for more nonpolar asids (benzoic, phenylpropanoic, cinnamic).
Разработка условий дериватизации фенилкарбоновых кислот, выделенных из сыворотки крови
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