Combination of planar chromatography with matrix-assisted laser desorption/ionization mass spectrometry for the analysis of biologically active compounds
T11N4
Combination of planar chromatography with matrix-assisted laser
desorption/ionization mass spectrometry for the analysis of biologically
active compounds
R.S. Borisov, D.I. Zhilyaev, C.A. Esparsa Sandoval, N.Yu. Polovkov, V.G. Zaikin
MALDI mass spectra of 33 pharmacological substances recorded from standard metal targets with the use of four different matrices (HABA, DHB, IAA and AT) have been obtained for the first time. They reveled only the peaks for ions [M+H]+, [M+Na]+ and [M+K]+ having different abundances. It has been shown that MALDI spectra of the compounds can be recorded as a result of desorption/ ionization of analytes from spots on thin-layer chromatogram (TLC) (stationary phase – silica gel) in the presence of the same tested matrices. It has been found that graphite can be efficiently used as a matrix in a combination of TLC and MALDI mass spectrometry because it eliminates the danger of erosion of spots on TLC plates during the treatment with matrix solution and reduces the time spent on sample preparation.
