Use of mass spectrometry for characterizing the growth of Pseudomonas bacteria bearing naphthalene degradation plasmids
T11N4
Use of mass spectrometry for characterizing the growth of Pseudomonas
bacteria bearing naphthalene degradation plasmids
A.M. Zyakun, V.V. Kochetkov, V.N. Zakharchenko, B.P. Baskunov, K.S. Laurinavichus,
V.P. Peshenko, T.V. Siunova, T.O. Anokhina, A.M. Boronin
Metabolites formed during the growth of Pseudomonas bacteria bearing various naphthalene degradation plasmids were identified using high performance liquid chromatography and mass spectrometry in combination with electrospray/ ionization. It was shown that a part of naphthalene consumed by growing bacteria P. aureofaciens BS1393(NPL-41) with plasmid NPL-41 was transformed in salicylic acid and phenol as main and minor exometabolites. The growth of P. aureofaciens BS1393(pOV17) bacteria bearing plasmid pOV17 was accompanied bycompletel oxidation of naphthalene and by a minor production of salicylic acid as exometabolites. On the basis of molecular mass spectrometry, we determined СО2 production dynamics and oxygen uptake in the process of bacterial growth. Specific production of СО2 per unit of cell amount during the growth of bacteria bearing plasmid NPL-41 exceeds five times the production of P. aureofaciens BS1393(pOV17). The respiratory quotient (RQ) representing the ratio of the molar concentrations of metabolic СО2 to consumed oxygen depends on the type of plasmids that determine naphthalene degradation. The value of RQ in the growth of P. aureofaciens BS1393(pOV17) bacteria was almost 1.5 times greater than in the case of P. aureofaciens BS1393(NPL-41). The uptake of О2 for production of СО2 was in the former case 4.5 mol О2/mol СО2; in the case of the latter culture, 6.5 mol О2/mol СО2.
