Determination of thiodiglycol and its oxide in biomedical samples by gas chromatography/mass spectrometry
T14N2
N.L. Koryagina, E.I. Savel’eva, N.S. Khlebnikova, A.S. Radilov
An unified procedure for the determination of thiodiglycol (TDG) and its oxide (TDGO) as markers of exposure to sulfur mustard (SM) in biomedical samples (urine, blood plasma, hairs) by means of gas chromatography/mass spectrometry with electron ionization was suggested. The detection limit of the procedure compares with the lowest level of endogenous TDG in biosamples (1 ng ml–1). Solid-phase microextraction of the derivatized analytes from the reaction mixture allows the reduction of the matrix effect. The procedure was used for the analysis of rat biosamples obtained after in vivo exposure to SM. The procedure was found to offer a higher potential in the retrospective determination of the total TDG and TDGO level in rat urine (within 7 days after exposure to 2 mg kg–1 SM) compared to the determination of the same markers of SM in blood plasma. Blood plasma can be used for confirmatory analysis, provided the samples are taken within 2 days after exposure. Hair were found to be the least suitable biomatrix in view of the variability of their background TDG level and the possibility of TDG contamination from other sources.
