Characterization of the structural forms of biomolecules based on the decomposition and separation of the charge state distributions of their ions

T15N3

V.V. Raznikov, M.O. Raznikova

The aim of this work is the efficient disentangling and analysis of hidden information in mass spectrometric data on the structural composition of the studied solutions of biomolecules in their electrospray ionization. This paper provides a description of the algorithm of the sequential splitting combined with a decomposition of the measured charge state distributions of biomolecules, while increasing the number of individual components corresponding to the independent presence of charge carriers with the found probabilities of retention for a given number of ionogenic groups of these biomolecules. The article discusses the results of calculations for two-dimensional and one-dimensional decomposition of the charge distributions of cytochrome с with separation, presumably corresponding to different conformations of proteins. For the first time it was developed a method and tool for searching individual components and their contributions to the mass spectra of multiply charged ions, when the mass spectra of these components are not known in advance.

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Disentangling of information about the structure of biomolecules based on decomposition and separation of two-dimensional charge distributions of ions 

T13N2

V.V. Raznikov, M.O. Raznikova, M.L. Pridatchenko Читать полностью

(Русский) Декомпозиция многомерных зарядовых распределений ионов. Часть 2

Sorry, this entry is only available in Русский.

(Русский) Декомпозиция многомерных зарядовых распределений ионов. Часть 1

Sorry, this entry is only available in Русский.