Comparative determination of fatty acid composition of low-molecular components of blood plasma by three mass spectrometry techniques: the ‘old-new’ exercise in lipidomics


B.L. Milman, V.A. Utsal′, N.V. Lugovkina, I.A. Kotryakhov,  I.K. Zhurkovich

Fatty acid composition of lipid low-molecular components of human blood plasma was determined by the combination of analytical techniques, such as gas chromatography/mass spectrometry and high-resolution electrospray MS1 and MS2 with direct flow injection. More than two tens of fatty acid methyl esters were identified in lipid extracts subjected to methanolysis. Direct injection electrospray MS led to recognition of 21 lysophosphatidylcholines and 25 phosphatidylcholine sum compositions. Fatty acid distributions over analytical signal intensities in groups of methyl esters and lysophosphatidylcholines were demonstrated to be similar to each other and highly correlated. The fatty acid distribution for phosphatidylcholine was correlated to those to a lesser extent. At the same time, the analytical signal intensities of lysophosphatidylcholines and phosphatidylcholine was observed to be highly correlated to concentrations of these lipid compounds in blood plasma determined by the similar technique of direct injection MS and available in the literature.

Читать полностью

Идентификация молекулярных видов церамидов эритроцитов

Sorry, this entry is only available in Русский.

(Русский) Применение высокоэффективной жидкостной хроматографии/масс-спектрометрии

Sorry, this entry is only available in Русский.