The «low-molecular» approach to identification of microorganisms by MALDI mass spectrometry
T13N3
B.L. Milman, Yu.Yu. Ilyasov, N.V. Lugovkina, A.A. Golovina1, A.V. Dmitriev
The new approach to microorganism identification is presented which includes the transformation of their MALDI mass spectra directed to decreasing mass scale by the order of magnitude and the use of the standard software for building mass spectral libraries of low-molecular compounds and library searches. The library of 728 transformed mass spectra of 182 strains for some Streptococcus species was built. With this library, the rate of true identification was estimated by cross-comparison between library mass spectra. The true identification rate is 84 % for three Streptococcus species that corresponds to the average trueness of species identification by MALDI as found in the literature. The proposed approach to identification can be considered as the method of choice for solving problems of bacteria identification.
