Some aspects of experimental design in the targeted proteomics based on using selected reaction monitoring and isotope-labeled peptides
T11N3
Some aspects of experimental design in the targeted proteomics based
on using selected reaction monitoring and isotope-labeled peptides
E.D. Virus, A.V. Ivanov, B.P. Luzyanin, A.A. Kubatiev
Institute of general pathology and pathophysiology RAMS,
125315, Moscow, Baltiyskaya st., 8, Russia,
E-mail: edwardvirus@yandex.ru
Selected reaction monitoring is more than 12 years one of preferred approaches of many mass spectrometrists in detecting the ultra-trace quantities of low-molecular weight substances. Today we observe active implementation of the given approach in proteomics, where it becomes powerful tool of quantitative proteomics. Targeted quantitative analysis with selected reaction monitoring provides the unique selectivity, ultrahigh sensitivity and a wide linear range. Presented paper familiarizes the reader with some aspects of experimental design for selected reaction monitoring in carrying out the proteomic experiments with the stable isotope dilution.
