Determination of flavonoids of Epimedium by high performance liquid chromatography with tandem mass spectrometric detection
T12N2
O.A. Shevlyakova, K.J. Vasil’yev, A.A. Ihalaynen, A.M. Antokhin,
V.F. Taranchenko, V.M. Goncharov, A.V. Aksenov, D.A. Mitrofanov,
I.A. Rodin, O.A. Shpigun
An approach for simultaneous detection of icariin, icaritin, icarisides I, II, epimedins A, B, C in extracts from plants and сommегсial pгoduсts was developed on the basis of liquid chromatography/mass spectrometry. Using the dry and liquid extracts of Epimedium and the tea, the validation of the approach was carried out. Limits of detection were 5×10−4 mkg ml−1. For compounds determination, electrospray ionization in positive ion mode and tandem mass spectrometric detection in multiple reaction monitoring mode were used.
