Profiling of phytoecdysteroids in extract of sawwort (Serratula coronata) using high-performance liquid chromatography / high resolution tandem mass spectrometry
T12N3
D.A. Sevko, M.K. Beklemishev, I.A. Rodin, A.A. Ihalaynen,
A.M. Antokhin, V.F. Taranchenko, V.M. Goncharov,
D.A. Mitrofanov, A.V. Aksenov
This paper reports the research of phytoecdysteroids in extract of sawwort (Serratula coronatа) by high-performance liquid chromatography coupled with high resolution mass-spectrometry. Mass-spectra of target analytes including tandem mass-spectra are obtained. Fragmentation of phytoecdysteroids negative ions, generated using electrospray ionization, is studied. Structures of target analytes are proposed. Quantitative assessment of identified compounds is carried out.
