Determination of methylphosphonic acid in human blood plasma by high-performance liquid chromatography tandem mass spectrometry
T13N4
T.M. Baygildiev, I.A. Rodin, A.N. Stavrianidi, A.V. Braun, O.A. Shpigun, I.V. Rybalchenko
Using high performance liquid chromatography combined with tandem mass spectrometric detection, an approach for the determination of the most stable nerve agent biomarker, methylphosphonic acid, in human blood plasma has been developed. The proposed method is based on the derivatization reaction of methylphosphonic acid with p-bromophenacyl bromide. Optimization of conditions for human plasma sample preparation, mass spectrometric detection conditiones and gradient elution program has been performed. The proposed approach has demonstrated satisfactory reproducibility and selectivity of the determination, limit of detection for methylphosphonic acid in human plasma was 3 ng ml−1.
