Improved procedure for tandem gas chromatography/mass spectrometry detection of the globin adduct of sulfur mustard
T14N4
N.L. Koryagina, M.D. Shachneva, A.I. Ukolov, E.I. Savel’eva, N.S. Khlebnikova, A.S. Radilov
Features of electron and chemical ionization mass spectra of derivatives of N-[2-[(hydroxyethyl)thio]ethyl]-DL-valine (НЕТЕ-Val)—a biomarker of sulfur mustard (SM) — recorded by GC-MS and GC-MS/MS were studied. The retention indices of the studied derivatives were measured. The optimized procedure of the GC-MS/MS (MRM mode) identification of НЕТЕ-Val was tested in the analysis of donor blood samples exposed in vitro to sulfur mustard. The linear range of the detector was 10 to 100 ng mL– and the detection limit was 10 ng mL–1 for SM.
