Selection of registration conditions and fragmentation study of peptide biomarker of sarin by high-performance liquid chromatography high-resolution mass spectrometry
T15N1
A.N. Stavrianidi, A.V. Braun, E.A. Stekolshchikova, T.M. Baygildiev, I.A. Rodin, I.V. Rybalchenko
Identification of protein biomarkers of chemical warfare agents requires their reliable detection in the blood plasma at trace levels. This is a challenging task in the modern liquid chromatography-mass spectrometry. Existing approaches are based on the formation of tyrosine adducts with alkylmethylphosphonic acids after the cleavage of all proteins. The structure of such adducts is related to the type of chemical agent used. In this study, the possibility of detecting the tripeptide adduct of isopropylmethylphosphonic acid, obtained by trypsinolysis of the albumin adduct with sarin, in human plasma was shown. The study of the fragmentation ways of this analyte made it possible to identify the characteristic features of dissociation that can be used to determine other biomarkers of the application of chemical weapon. The conditions for recording the most intense ion transitions for rapid screening of blood plasma samples for the content of the albumin adduct of sarin with trypsinolysis according to the filter aided sample preparation method are described.
