Study of the nettle (Urtica diоica) lignin by orbitrap mass spectrometry with atmospheric pressure photoionization
T16N1
I.I. Pikovskoi, D.S. Kosyakov, I.S. Shavrina, N.V. Ul’yanovskii
The orbital ion trap high-resolution mass spectrometry method with atmospheric pressure acetone-doped photoionization was first used to characterize the structure of lignins of herbaceous plants using the example of nettle (Urtíca dióica) dioxane lignin. The obtained mass spectrum contains about 3,000 peaks of deprotonated molecules of lignin oligomers in the molecular mass range up to 1.6 kDa. The study of tandem mass spectra and the composition of monomers formed during the collision-induced dissociation of lignin macromolecules showed the special role of p-hydroxycinnamic acids in the formation of nettle lignin. Based on the results of tandem mass spectrometry, possible structures of nettle lignin oligomers, which are formed by the addition of guaiacyl- and syringylpropane units with their subsequent etherification with p-coumaric, ferulic and dihydroferulic acids, are proposed.