Simultaneous determination of wogonin, scutellarin, baicalin and baicalein in the extracts from Scutellariae baicalensis by high performance liquid chromatography with tandem mass spectrometry
T15N1
D.I. Baygildieva, T.M. Baygildiev, A.N. Stavrianidi, O.A. Shpigun, I.A. Rodin
Scutellariae baicalensis is one of the most popular herb in the traditional Chinese medicine. It is used for the treatment of inflammatory process, hypertension, cardiovascular diseases, and also in treatment of bacterial and virus infections due to such active components as wogonin, scutellarin, baicalin and baicalein that are contained in this herb. A sensitive and selective technique of simultaneous determination of these compounds in the extracts from plants raw materials using high performance liquid chromatography tandem mass spectrometry with electrospray ionization was developed. The proposed method has been successfully tested on the commercially available samples of Scutellariae baicalensis root. Analysis of samples was carried out using a reversed-phase chromatography with Acclaim RSLC C18 sorbent. Using multiple reactions monitoring the following limits of detection were achieved: 1 ng mL–1 for wogonin and baicalin, 3 ng mL–1 for scutellarin and 4 ng mL–1 for baicalein. It was found that calibration curve was linear in the concentration range of 20 ng mL–1 and 2000 ng mL–1 for scutellarin and 20 ng mL–1 and 500 ng mL–1 for wogonin, baicalin and baicalein.
Хроматомасс‑спектрометрическое определение среднелетучих органических веществ в конденсате выдыхаемого воздуха
Sorry, this entry is only available in Русский.
Одновременное определение салидрозида, розарина и розавина в экстрактах из Rhodiola rosea
Sorry, this entry is only available in Русский.
