Determination of the geographical region of growing the coffee beans by gas chromatograph-combustion – isotope ratio mass spectrometry
T13N3
A.M. Antokhin, V.F. Taranchenko, S.V. Vasilevsky, A.V. Aksenov, Ju.B. Aksenova, O.V. Kuznetsova,
I.V. Lapko, M.A. Glushkova
Gas chromatography –combustion –isotope ratio mass spectrometry has been used to study the changes in carbon isotopic composition of chlorogenic acid and caffeine extracted from samples of coffee beans of different origin. It has been shown that depending on the region of coffee beans growing, the relative characteristics of carbon isotopic composition, namely δ13C, ‰, changed significantly for both caffeine and chlorogenic acid. There was a decrease in the 13C isotope observed in the following order: Africa –South America –Central America. It has been found that the δ13C values for caffeine and chlorogenic acid, when mutually considered, provide the statistically significant identification of a region where the coffee beans grow.
