Predictive liquid chromatography of peptides based on hydrophilic interactions for mass spectrometry-based proteomics
T14N2
A.A. Lobas, L.I. Levitsky, A. Fichtenbaum, A.K. Surin, M.L. Pridatchenko, G. Mitulović,
A.V. Gorshkov, M. V. Gorshkov
High-performance liquid chromatography (HPLC) is widely used for separation of complex peptide mixtures before mass spectrometry-based proteome analysis. In such analysis, reversed phase HPLC (RP-HPLC) using non-polar stationary phases such as surface-modified silica containing alkyl groups (e.g., C18) is typically employed. Because of the high heterogeneity of proteomic samples, multidimensional separation approaches gained increasing attention recently to tackle this complexity and extremely high range of concentrations. In two-dimensional liquid chromatography, hydrophilic interaction chromatography (HILIC) is often a method of choice for combination with RP-HPLC because it uses reversed-phase type of eluents and allows efficient separation of polar peptides. Due to the high degree of orthogonality in this two-dimensional separation space, it is tempting to develop approaches for predicting peptide retention times for HILIC-based separations similar to the ones for RP-HPLC. Recent successful efforts in this area were focused on developing retention coefficient (RC)-based approaches. Herein, we explored the feasibility of using a statistical thermodynamic model for prediction of peptide retention times in HILIC separations and determined the phenomenological parameters of the model for a bare silica column. The performance of the developed model was tested using HPLC-MS analysis of a set of synthetic peptides, as well as a tryptic peptide mixture.
GroupFilter: a software tool for efficient filtering of Morpheus search engine results
T12N4
M.V. Ivanov, L.I. Levitsky, A.A. Lobas, M.V. Gorshkov Читать полностью
Peptide identifications in «shotgun» proteomics using tandem mass spectrometry
T12N1
Peptide identifications in «shotgun» proteomics using tandem
mass spectrometry: comparison of search engine algorithms
M.V. Ivanov, L.I. Levitsky, A.A. Lobas, I.A. Tarasova, M.L. Pridatchenko,
V.G. Zgoda, S.A. Moshkovskii, G. Mitulovic, M.V. Gorshkov
Pepxmltk — a format converter for peptide identification results obtained
T11N3
Pepxmltk — a format converter for peptide identification results
obtained from tandem mass spectrometry data using X!Tandem
search engine
M.V. Ivanov1,2, L.I. Levitsky1,2, I.A. Tarasova1, M.V. Gorshkov1,2 Читать полностью
Высокоэффективный анализ и идентификация пептидов и белков
Sorry, this entry is only available in Русский.
Альтернативные способы проверки масс-спектрометрических методов идентификации пептидов
Sorry, this entry is only available in Русский.
Инверсия хроматографических порядков выхода пептидов
Sorry, this entry is only available in Русский.
Картирование протеома опухолевого плеврального выпота
Sorry, this entry is only available in Русский.
