Detection of metabolites of TMCP-CHMINACA, the new psychoactive substance, in rat urine and serum by liquid chromatography-mass spectrometry
T15N3
E.V. Nikitin, A.M. Grigoryev, A.V. Labutin, S.E. Gribkova, I.A. Rodin, V.A. Kalashnikov, K.R. Ahmerov
The appearance of the new psychoactive substance TMCP-CHMINACA [3- (2,2,3,3-tetramethylcyclopropane-1-oyl)-1-cyclohexylmethyl-1H-indole] on the market necessitated the determinftion of its chromatomass spectrometry characteristics as well as the search of its metabolites for further addition of their mass spectra to a library, used in screening of biological objects in forensic science. The structure of the molecule TMCP-CHMINACA has a significant similarity to the previously known synthetic cannabinoid TMCP-018 and TMCP-2201. High-resolution liquid chromatographic analysis of rats urine and blood serum after intravenous injection of the TMCP-CHMINACA solution showed metabolites that are the products of tetramethylcyclopropane residue carboxylation, N-dealkylation, and additional hydroxylyoxylation compounds of indole and tetramethylcyclopropane residues. Using chromatographic and mass spectrometry characteristics of TMCP-CHMINACA and its metabolites obtained from the study of rat biological fluids, a number of metabolites of both TMCP-CHMINACA and its isomer formed during smoking were found in urine studies of three people. At the same time, an unchanged TMCP-CHMINACA was detected on the surface and in the inner area of the hair sample of one of the participants. It has been established that an important way of biotransformation of TMCP-CHMINACA in both humans and rats is the carboxylation of the tetramethylcyclopropane residue combined with the monohydroxylation of the cyclohexylmethyl residue. For rats, the formation of N-dealkylation products combined with the monohydroxylation of the tetramethylcyclopropane residue is more typical. The presented mass spectra and retention characteristics of the detected metabolites can help in the detection of these (or similar) compounds in human biological objects.
Detection of metabolites of furanylfentanil, the new psychoactive substance, in rat urine and serum by liquid chromatography/mass spectrometry
T14N4
I.A. Rodin, S.E. Gribkova, A.M. Grigoryev, E.V. Nikitin, V.A. Kalashnikov
The appearance of new psychoactive substance furanilfentanil [N-phenyl-N-(1-(2-phenylethyl) piperidin-4-yl) furan-2-carboxamide] in the market necessitated the determination of its chromato-mass spectrometric characteristics, as well as its metabolites, for the subsequent automated control of them in biological objects. The structure of the furanylfentanyl molecule has a significant similarity with fentanyl and its structural analogues. This article describes the detection of metabolites of furanylfentanil in urine in laboratory rats and presents data on their proposed structures. Using high-resolution liquid chromatography/mass spectrometry, a number of metabolites were discovered in urine. Among them there are the products of monohydroxylation of phenylethane and N-phenyl residues; dihydroxylation and dihydroxylation combined with methylation of the phenylethane; formation of dihydrodiol and additional hydroxylation; N-dealkylation; hydrolysis of the amide bond and additional hydroxylation. Using chromatography/mass spectrometry characteristics of furanilfentanil and its metabolites which were founded in rats urine earlier, a number of them as well as furanilfentanil itself were also discovered in postmortem blood and urine samples of humans. It has been established that the main route of biotransformation of furanylfentanyl, in both humans and rats, is a monohydroxylation of the phenylethyl fragment, formation of dihydrodiol and hydrolysis. Presented mass spectra and retention characteristics of founded metabolites can help in the detection of these (or corresponding compounds) in biological human fluids
Detection of metabolites of flavonoids Epimedium in rats urine by high performance liquid chromatography-high-resolution tandem mass spectrometry
T13N2
O.A. Shevlyakova, K.J. Vasil’yev, A.A. Ihalaynen, A.M. Antokhin, V.F. Taranchenko,
V.M. Goncharov, A.V. Aksenov, D.A. Mitrofanov, I.A. Rodin, O.A. Shpigun Читать полностью
Non-target screening of markers of synthetic cannabinoids in urine using HPLC-MS/MS
T12N1
Non-target screening of markers of synthetic cannabinoids in urine
using HPLC-MS/MS
A.V. Labutin, A.Z. Temerdashev
Detection and identification of trace amounts of chlorine organic substances
T11N1
Detection and identification of trace amounts of chlorine organic
substances by gas chromatography-high-resolution mass spectrometry
A.А. Shelepchikov*, E.S. Brodsky
